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(A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
Mem Non Essential Amino Acids Solution (Neaa) 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mem non-essential amino acids solution (neaa) 100/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mem non-essential amino acids solution (neaa) 100 - by Bioz Stars, 2026-03
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mem non-essential amino acid solution 100  (Thermo Fisher)
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(A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

Journal: Bio-protocol

Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)

doi: 10.21769/BioProtoc.5304

Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

Techniques: Derivative Assay, Control, Staining, Marker, Expressing

Journal: Bio-protocol

Article Title: Isolation and Culture of Primary Pericytes from Mouse

doi: 10.21769/BioProtoc.5288

Figure Lengend Snippet:

Article Snippet: MEM non-essential amino acids (100×) (Thermo Fisher Scientific, catalog number: 11140050) 16.

Techniques: Concentration Assay